General description
2′-脱氧鸟苷5′-三磷酸(dGTP)的结构为鸟嘌呤碱基连接脱氧核糖,糖的5′-羟基位再连接三个磷酸残基链。可用于通过DNA聚合酶合成DNA。
dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.
Application
2′-脱氧鸟苷5′-三磷酸三钠盐可用于DNA测序、PCR和其他基于DNA聚合酶的DNA合成和修复技术。
Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
Analysis Note
Typical analysis:
- ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
- Absorbance determination A250/A260, A280/A260, A290/A260
- DNase activity negative, according to the current Quality Control procedures
- Suitability for PCR
Other Notes
1 μmol dGTP = 13.7 A252 units
For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
- nucleotide sets (all 4 nucleotides in separate vials)
- ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
- kits (dNTPs, buffer, and polymerase in separate vials)
- ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]
| description | Lithium salt |
| assay | 98% |
| form | solution |
| usage | sufficient for 6,250 standard reactions (PCR) |
| packaging | pkg of 250 μL (25 μmol; 100 mM) |
| manufacturer/tradename | Roche |
| concentration | 100 mM |
| technique(s) | DNA amplification: suitable,DNA sequencing: suitable,DNA synthesis: suitable,PCR: suitable |
| storage temp. | −20°C |
| SMILES string | [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1 |
| InChI | 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1 |
| InChI key | IWGGLKOTEOCWQP-BIHLCPNHSA-K |

