General description
Yaddle1 is designed to interact specifically with the Piezo1 channel. It features a trifluoromethyl group, which is crucial for its agonistic activity.
Application
Yaddle1 may be used in immunology as a vaccine adjuvant, targeting optimal T cell activation, enhancing vaccine efficacy. It may also be used to study the role of piezo-type mechanosensitive ion channel component 1 in inflammatory bowel disease.
Biochem/physiol Actions
Yaddle1 is a mechanosensitive ion channel Piezo1 agonist with higher potency (Ca2+ influx EC50/Emax = 0.40 μM/2.6-fold using Piezo1 mCherry-expressing HEK293A) and greatly improved aqueous solubility (26.7 μM at pH 7.4) than Yoda1 (EC50/Emax = 0.50 μM/2.4-fold; solubility = 1.2 μM at pH 7.4). Yaddle1 binds to Piezo1 channel, stabilizing the open conformation of the Piezo1 channel and lowering the mechanical activation threshold.
| Quality Segment | 100 |
| assay | ≥98% (HPLC) |
| form | powder |
| color | white to beige |
| solubility | DMSO: 2 mg/mL, clear |
| storage temp. | 2-8°C |
