General description
Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Preparation Note
Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
| Quality Segment | 100,200 |
| form | liquid |
| usage | 200 uses (0.1 mg size),40 uses (20 μg size) |
| suitability | suitable for electrophoresis (DNA) |
| storage temp. | −20°C |
