产品详情

ʟ-谷氨酰胺合成酶来源于大肠杆菌，100UNITS，G1270-100UN，Sigma

销售价： ¥ 6284.33 / 件

优惠：请询价

订货号： 0BP8776

品牌型号： G1270-100UN

货期：询货期

最小订货量： 1件

产品介绍 Product Description

General description

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

Application

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

Biochem/physiol Actions

Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
谷氨酸降解酶

Physical form

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

Other Notes

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

技术参数 Specifications

form	lyophilized powder
Quality Segment	200
specific activity	400-2,000 units/mg protein
purified by	affinity chromatography
contains	dithioerythritol as preservative
composition	Protein, ~5% Lowry
solubility	H₂O: soluble 0.95-1.05 mg/mL, clear to hazy
UniProt accession no.	P0A9C5
foreign activity	ATPase <0.2%
storage temp.	−20°C
Gene Information	Escherichia coli K12 ... glnA(948370)

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