General description
Collagen alpha-1(II) chain (UniProt: P02458; also known as Alpha-1 type II collagen) is encoded by the COL2A1 gene (Gene ID: 1280) in human. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Collagens contain a Gly-X-Y repeating structure. The glycine residue is necessary in every third position to ensure close packaging. Although any amino acid can be present at position X or Y, proline is more frequently found in the X position and hydroxyproline in the Y position. Both proline and hydroxyproline stabilize the helical conformation of polypeptide chains. This triplet of amino acids allows collagen chains to twist into a helical structure ( -helix) forming a super helix. Each collagen molecule contains 3 chains twisted around each other to form a triple helix. These 3 chains may either be identical or different depending on the type of collagen. Type II collagen is a major constituent of cartilaginous tissues, representing 90 95% of its total collagen content and forming the fibrils that give cartilage its tensile strength. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. It is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 26-181), which are subsequently cleaved off to produce mature collagen. Cleavage at 181-182 residues is brought about by N-endopeptidases. Excessive cleavage of type II collagen by collagenases is observed in cases of osteoarthritis (OA). This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cahue, S., et al.(2007). Osteoarthritis Cartilage. 15(7); 819-823; Poole, AR., et al. (2002). Ann. Rheum. Dis. 61(Suppl II); 78-81).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Collagen type II.
Application
Quality Control Testing
Evaluated by Western Blotting on Pepsin digest of Chicken sternal collagen Type II.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Pepsin digest of Chicken sternal collagen Type II.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Collagen Type II in Human chondrocytes.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collagen Type II in Human cartilage tissue sections.
Flow Cytometry Analysis: 1 μg from a representative lot detected Collagen Type II in one million Human chondrocytes.
Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected purified Human Collagen Type II.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting on Pepsin digest of Chicken sternal collagen Type II.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Pepsin digest of Chicken sternal collagen Type II.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1K11, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 141.79 kDa,observed mol wt ~140 kDa |
| purified by | using Protein A |
| species reactivity | human, chicken |
| species reactivity (predicted by homology) | equine, porcine, monkey, bovine, feline, canine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_001835.3 |
| UniProt accession no. | P02458 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... COL2A1(1280) |
