General description
The gene IGFBP-2 (insulin-like growth factor binding protein-2) is mapped to human chromosome 2q35.
Biochem/physiol Actions
During development, insulin-like growth factor binding protein-2 is expressed in a number of tissues with the highest expression level found in the central nervous system. IGFBP-2 exhibits a 2-10 fold higher affinity for IGF II than for IGF I. Mouse and human IGFBP-2 share approximately 82% homology.
Exhibits a 2-10 fold higher affinity for IGF II than for IGF I.
IGFBP-2 (insulin-like growth factor binding protein-2) regulates the function of IGF ligands. IGFBP2 is associated with skeletal muscle cell proliferation and differentiation. It also plays an important role in the differentiation of corneal fibroblast.
Physical form
Lyophilized from a 0.2 μm filtered solution of 30% (v/v) acetonitrile with 0.1% (v/v) TFA
Analysis Note
The biological activity is measured by its ability to inhibit recombinant human lGF-II on MCF-7 cells.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in NSO cells |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.04 μg/mL |
| mol wt | ~36 kDa by SDS-PAGE,calculated mol wt ~31 kDa |
| packaging | pkg of 25 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P18065 |
| storage temp. | −20°C |
| Gene Information | human ... IGFBP2(3485) |
