General description
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgE myeloma protein. IgE (Immunoglobulin E) is the least abundant immunoglobulin isotype. IgE is produced by plasma cells and is highly functional in the lung, skin, and mucous membranes. Normal levels of IgE in serum are less than 1 μmL.
Immunogen
Purified human IgE
Application
Anti-Human IgE-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
IgE plays a critical role in allergic reactions and host protection from parasites. During an allergic reaction such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease, or primary immunodeficiency the serum IgE levels can be induced up to 5-fold. IgE specifically and reversibly binds to the membrane receptor Fc-epsilon receptors (FcεR) type I on the surface of basophils, eosinophils, and mast cells. This binding activates the release of histamine, leukotrienes, and other mediators responsible for hypersensitivity. The recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.
Monoclonal Anti-Human IgE specifically recognizes Human IgE. The antibody shows no cross-reactivity with human IgG1, IgG2, IgG3, IgG4, IgA, and IgM.
Physical form
Supplied as a lyophilized powder.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | peroxidase conjugate |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | GE-1, monoclonal |
| form | lyophilized powder |
| species reactivity | human |
| technique(s) | ELISA: 1:20,000-1:30,000 using 5 μg/mL Human IgE myeloma for coating |
| isotype | IgG2b |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
