General description
Interleukin-1 receptor-associated kinase 1 (UniProt: P51617; also known as EC:2.7.11.1, IRAK-1) is encoded by the IRAK1 (also known as IRAK) gene (Gene ID: 3654) in human. The IRAK family is composed of IRAK-1, -2, and -4, which are expressed in a variety of human immune cell types and IRAK-M whose expression is largely limited to monocytes and macrophage. IRAK-1 is a ubiquitously expressed serine/threonine-protein kinase that play a central role in inflammatory responses by regulating the expression of various inflammatory genes in immune cells. These signals are critical for elimination of viruses, bacteria, and cancer cells, as well as for wound healing. IRAK-1 is involved in Toll-like receptor (TLR) and IL-1R signal transduction pathways. Is rapidly recruited by MYD88 to the receptor-signal transduction complex upon TLR activation. Following recruitment on the activated receptor complex, it is phosphorylated on Thr-209, probably by IRAK-4, resulting in a conformational change of the kinase domain (aa 212-521) that allows further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity. IRAK-1 signal transduction is considered to be important in the development and progression of cancer. Helicobacter pylori induced gastric inflammation is reported to up-regulate TLR2 and TLR5 expression, which in turn increases IRAK-1 phosphorylation and activation and NF- B activation. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jain, A., et al. (2014). Front. Immunol. 5; 553; Kollewe, C., et al. (2004). J. Biol. Chem. 279(7); 5227-5236).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the internal region of human Interleukin-1 receptor-associated kinase 1 (IRAK-1).
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human colon tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected IRAK-1 in human colon tissue sections.
Tested applications
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected IRAK-1 in K562 cells.
Western Blotting Analysis: A 1:250 dilution from a representative lot detected IRAK-1 in HEK293 cell lysate.
Affinity Binding Assay:: A representative lot of this antibody bound IRAK-1 with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 3B12 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Interleukin-1 receptor-associated kinase 1 (IRAK-1). It targets an epitope within 24 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3B12, recombinant monoclonal |
| description | 3B12 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 76.54 kDa,observed mol wt ~70 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_001560 |
| UniProt accession no. | P51617 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... IRAK1(3654) |
