Application
Useful for Western blot.
Biochem/physiol Actions
Recombinant human STK9 (CDKL5) (1-498) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2).
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| mol wt | ~84 kDa |
| concentration | 0.1 μg/μL |
| accession no. | NM_003159 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... STK9(6792) |
