General description
Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.
Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.
Application
The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.
Biochem/physiol Actions
This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.
Packaging
1mg/ml in Plastic
Preparation Note
Store product at -20°C. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.
Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.
Reconstitute in sterile water.
Other Notes
For R&D only.
| biological source | Achromobacter lyticus |
| Quality Level | 100,200 |
| recombinant | expressed in E. coli (206-473aa) |
| grade | Proteomics Grade |
| form | lyophilized powder |
| optimum pH | 8.5 |
| pH range | 9.0-9.5(catalytic) |
| UniProt accession no. | P15636 |
| storage temp. | −20°C |
