M-MLV 逆转录酶，40000UNITS，M1302-40KU，Sigma

General description

莫洛尼鼠白血病病毒(M-MLV)逆转录酶从质粒表达M-MLV部分pol基因的大肠杆菌中分离获得。MoMLVRT由671个氨基酸组成。它是一种DNA聚合酶，可通过单链RNA、DNA或RNA-DNA杂合体（使用引物）合成互补的DNA链。

Application

M-MLV逆转录酶已用于：

• 制备cDNA文库或合成**条cDNA链
• 2步法RT-PCR检测
• 实时定量聚合酶链反应(RT-qPCR)
• 逆转录

Features and Benefits

• 热稳定性逆转录酶在37℃时活性佳。
• 可用于制备长达7 kb的**链cDNA。

Packaging

本品为含DTT 的10× M-MLV逆转录酶缓冲液。

Preparation Note

该酶从可在质粒上表达M-MLV pol基因的Escherichia coli 中纯化所得。

Other Notes

一单位本品可在10分钟内将1 nmol TTP整合进酸性可沉淀物质中。反应在37℃下进行，以poly(A)：oligo dT为模板：引物。

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.