Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human tag-free Tau-316 was expressed in E. coli cells. Tau-316 encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. Tau-316 transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type (1). Tau-316 gene mutations have been associated with several neurodegenerative disorders such as Alzheimer′s disease, Pick′s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy (2).
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Other Notes
Full Length
| recombinant | expressed in E. coli |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| mol wt | ~39 kDa |
| concentration | 0.2 μg/μL |
| accession no. | P10636-3 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... MAPT(4137) |
