General description
Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
| biological source | human |
| recombinant | expressed in baculovirus infected insect cells |
| assay | ≥60% (SDS-PAGE) |
| form | aqueous solution |
| specific activity | ≥1770 pmol/min-μg |
| mol wt | 129 kDa (p110α), 88 kDa (p85α) |
| packaging | pkg of 20 μg |
| concentration | 0.38 mg/mL |
| NCBI accession no. | U79143 (p110alpha),XM_043865 (p85alpha) |
| UniProt accession no. | P42336 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PIK3CA(5290) |
