General description
IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. Elevated levels of IL-5 lead to Eosinophilia, which may result in the induction of asthma and other allergic diseases. Human and murine IL-5 is cross-species reactive. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.
Biochem/physiol Actions
IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.
Physical form
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium glycinate, pH 8.5
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | ≤0.25 ng/mL ED50 |
| mol wt | 26.0 kDa |
| packaging | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | off-white to yellow |
| UniProt accession no. | P05113 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... IL5(3567) |
