General description
Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the HP-6017 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG myeloma proteins covalently coupled to poly aminostyrene (PAS) microbeads. IgGs (Immunoglobulin G) are the most common Immunoglobulin′s isotype in blood, lymph, cerebrospinal and peritoneal fluids and serve as key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), consist of a variable Fab fragment (which includes the antibody recognition site) and a conserved Fc fragment.
Immunogen
Human IgG myeloma proteins
Application
Anti-Human IgG (Fc specific)-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
IgG (Immunoglobulin G) deficiencies are often associated with various diseases. The Fc fragment has various important functions (e.g complement fixation, site for rheumatoid factor attachment and protein A binding) which indicate on the importance of immunoreagents specific for the Fc fragment of Human IgG.
Physical form
Supplied as a lyophilized powder.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | peroxidase conjugate |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | HP-6017, monoclonal |
| form | lyophilized powder |
| species reactivity | human |
| concentration | ~2 mg/mL |
| technique(s) | ELISA: 1:80,000-1:160,000 using 5 μg/mL Human IgG myeloma for coating |
| isotype | IgG2a |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
