General description
GPX1 (glutathione peroxidase 1) is a selenoprotein and the gene is mapped to human chromosome 3p21.3. It is present in the cytoplasm and is widely expressed.
Biochem/physiol Actions
GPX1 (glutathione peroxidase 1) is responsible for scavenging free radicals and derivatives. GPXs reduces lipid hydroperoxides to the corresponding alcohols as well as free hydrogen peroxide to water. Mutations in the GPX1 gene are associated with nonalcoholic fatty liver disease (NAFLD) susceptibility. Mutation in GPX1 at C594T is linked with oxidative stress-associated disorders, including prostate cancer and bladder cancer.
Physical form
Lyophilized from 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl, 1 mM DTT.
Preparation Note
Reconstitute with 100 μL deionized water.
Other Notes
Human GPX1 (aa 1-201) is fused at the C-terminus to a His-tag.
| biological source | human |
| recombinant | expressed in E. coli |
| tag | His tagged |
| assay | >90% (SDS-PAGE) |
| form | lyophilized |
| mol wt | ~25 kDa by SDS-PAGE |
| packaging | pkg of 10 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| impurities | <1 EU/μg endotoxin, tested |
| color | white |
| UniProt accession no. | P07203 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... GPX1(2876) |
