Anti-XPO5 antibody produced in rabbit，100UL，HPA018402-100UL，Sigma

General description

Exportin-5 (XPO5) belongs to karyopherin-β protein family. The protein shuttles between the nucleus and the cytoplasm.

Immunogen

Exportin-5 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

Exportin-5 (XPO5) participates in the regulated nuclear export of RNAs and RNA binding proteins. It is involved in nuclear export of interleukin enhancer binding factor (ILF)-3 via RNAs containing minihelix motif in RanGTP dependent manner. Another nuclear export substrate of XPO5 is translation elongation factor 1A (eEF1A). eEF1A binds XPO5 via aminoacylated tRNAs. Interestingly, XPO5 is also involved in nuclear export of pre-miRNAs in RanGTP dependent manner. Depletion of XPO5 results in reduced miRNA levels. XPO5 is important for nuclear export of dicer mRNA, which is required for pre-miRNA maturation. XPO5 is required for nuclear export of 60S subunits in human cells. Single nucleotide polymorphism in XPO5 is associated with increased primary ovarian insufficiency risk. XPO5 is up-regulated in bladder urothelial carcinoma. Silencing XPO5 inhibits cells proliferation and apoptosis in bladder urothelial carcinoma cells.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Other Notes

Corresponding Antigen APREST73959

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.