General description
Transcriptional repressor CTCF (UniProt: P49711; also known as 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog) is encoded by the CTCF gene (Gene ID: 10664) in human. CTCF is a ubiquitously distributed chromatin binding factor that binds to DNA sequence specific sites. It contains 11 C2H2-type zinc finger regions. CTCF is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. CTCF serves as a transcriptional repressor by binding to promoters of vertebrate MYC gene and BAG1 gene. It can also serve as a transcriptional activator of APP. It also plays a critical role in gene silencing over considerable distances in the genome and preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. When bound to different DNA sequences, it can dimerize to mediate long-range chromatin looping. CTCF undergoes sumolyation on lysine 74 and 689, which contributes to its repressive function on the MYC P2 promoter. Mutations in CTCF gene are known to cause mental retardation characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from C-terminal region of human Transcriptional repressor CTCF.
Application
Anti-CTCF, clone 1B18 ZooMAb, Cat. No. ZRB1009, is a recombinant Rabbit Monoclonal Antibody that specifically targets Transcriptional repressor CTCF and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CTCF in NIH3T3 and C6 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CTCF in HUVEC, HeLa, A431, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CTCF in human prostate cancer tissue sections.
Biochem/physiol Actions
Clone 1B18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Transcriptional repressor CTCF. It targets an epitope with in 17 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1B18, monoclonal,recombinant monoclonal |
| description | 1B18 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 82.79 kDa,observed mol wt ~120 kDa |
| species reactivity | human, mouse, rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable using 1:100,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100,western blot: suitable using 1:1,000 |
| isotype | IgG |
| UniProt accession no. | P49711 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CTCF(10664) |
