General description
TPO (thrombopoietin) is a hematopoietic cytokine expressed in multiple tissue types. It is predominantly produced by liver and kidney and acts as a ligand for the cell surface receptor c-mpl.
Biochem/physiol Actions
TPO (thrombopoietin) is the key regulator of thrombopoiesis for platelet lineage. In both mice and humans, inactivation of this gene results in significant decrease in number of mature platelets produced by marrow progenitors. Transgenic mice with increased expression of this protein show progressive fibrosis and osteosclerosis of the bone marrow, as well as histological changes in kidneys, leading to mesangioproliferative glomerulopathy.
Physical form
Lyophilized from 10 mM Tris, pH 8.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
| biological source | mouse |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | ≥1.0 ng/mL ED50 |
| mol wt | 18.7 kDa |
| packaging | pkg of 10 μg |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white |
| UniProt accession no. | P40226 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | mouse ... Thpo(21832) |
