General description
It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.
Biochem/physiol Actions
Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. Recombinant human IL-33 is a 17.9 kDa protein containing 159 amino acid residues.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 8.0 + 0.5 mM DTT.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | ≥0.05 ng/mL ED50 |
| mol wt | 17.9 kDa |
| packaging | pkg of 10 μg |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white to off-white |
| UniProt accession no. | O95760 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... IL33(90865) |
