General description
Cluster of differentiation 140a (CD140a), also known as platelet-derived growth factor receptor α(PDGFRA), is encoded by the gene mapped to human chromosome 4q12. The encoded protein belongs to the receptor tyrosine kinase gene family.
The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.
Immunogen
CD140a-transfected NIH 3T3 cells
Application
The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (0.25 mL) is sufficient for 25 tests.
Biochem/physiol Actions
Platelet-derived growth factor receptor plays a vital role in the development and maturation of platelets. Cluster of differentiation 140a (CD140a)/ PDGFRA acts as a potential target of for imatinib, a revolutionary drug for the treatment of chronic myeloid leukemia. PDGFRA pathway might be involved in developmental process of thrombocytes. The encoded protein facilitates hepatic stellate cell (HSCs) proliferation and migration. Mutation in the gene has been observed in gastrointestinal stromal tumors (GISTs).
Features and Benefits
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Physical form
Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | Allophycocyanin conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 16A1, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_006206.4 |
| UniProt accession no. | P16234 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PDGFRA(5156) |
