General description
Anti-PKD1 Antibody detects endogenous levels of total PKD1 protein.
Polycystic kidney disease 1/polycystin-1 (PKD1/PC1) gene comprises of 46 exons and spans approximately 52 kb of genomic sequence. Polycystin-1 (PC1) is expressed at high levels in the brain and moderately in the kidney in adult tissue. It is present in the primary cilium of most mammalian cells. PC1 is also located at focal adhesions, desmosomes, and adherens junctions in the plasma membrane. Anti-PKD1 antibody detects endogenous levels of total PKD1 protein.
Immunogen
The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu.
Immunogen Range: 171-220
Application
Anti-PKD1 antibody produced in rabbit has been used in western blot analysis(1:500).
Biochem/physiol Actions
Polycystic kidney disease 1/polycystin-1 (PKD1/PC1) plays a regulatory role in cell migration and cell proliferation through mammalian targets of rapamycin (mTOR) and Janus kinase (JAK) signals. PKD1 inhibits cell migration and in some situations, acts as a tumor suppressor protein in the lung cancer cell line (A549). Before birth, polycystin-1 is the most active protein in kidney cells, and it interacts and works with polycystin-2 (PKD2). Mutations in the PKD1 gene are responsible for 85 percent of polycystic kidney diseases (PKDs).
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen 101 kDa |
| species reactivity | mouse, human, rat |
| concentration | ~1 mg/mL |
| technique(s) | ELISA: 1:10000,immunofluorescence: 1:100-1:500,immunohistochemistry: 1:50-1:100,western blot: 1:500-1:1000 |
| NCBI accession no. | Q15139 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... KPCD1(5587) |
