General description
Anti-Cellular Fibronectin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the FN-3E2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the antigens released in culture from a breast cancer cell line. Fibronectin (FN), also known as cold-insoluble globulin (CIG), is an extracellular matrix multi-domain glycoprotein composed of two nearly identical disulphide bound polypeptides. Fibronectin 1 is a glycoprotein, that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Cellular fibronectin is produced by various cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells, and myocytes.
Immunogen
Antigens released in culture from a breast cancer cell line
Application
Anti-Cellular Fibronectin antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Anti-cellular Fibronectin antibody, mouse monocloneal has been used in various immunochemical techniques including immunoblotting (220-260 kDa), immunofluorescence, and immunohistochemistry.
Biochem/physiol Actions
Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, haemostasis and thrombosis. It is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism.
Physical form
The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains fetal calf serum.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | FN-3E2, monoclonal |
| form | buffered aqueous solution |
| mol wt | ~220-260 kDa |
| species reactivity | chicken, rat, human, mouse |
| technique(s) | immunoblotting: suitable,immunofluorescence: 1:2,000-1:4,000 using human foreskin fibroblast Hs68 cells,immunohistochemistry: suitable |
| isotype | IgM |
| UniProt accession no. | P02751 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... FN1(2335) |
