产品介绍
Product Description
General description
Human DOT1L, GenBank Accession No. NM_032482), amino acids 2-416 with N-terminal ST tag, MW = 73 kDa, expressed in E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
技术参数
Specifications
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 73 kDa |
| packaging | pkg of 50 μg |
| concentration | >0.02 mg/mL |
| NCBI accession no. | NM_032482 |
| UniProt accession no. | Q8TEK3 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... DOT1L(84444) |
