产品介绍
Product Description
General description
Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Preparation Note
φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
技术参数
Specifications
| Quality Level | 100,200 |
| form | liquid |
| usage | 200 uses (0.1 mg size),40 uses (20 μg size) |
| suitability | suitable for electrophoresis (DNA) |
| storage temp. | −20°C |
