蛋白酶 K 来源于林伯氏白色念球菌，1G，SRE0047-1G，Sigma

General description

蛋白酶 K 是一种细胞外内肽酶，模具 Tritirachium album Limber 合成。蛋白酶 K 属于枯草杆菌蛋白酶的一个新亚家族。是由 277 个氨基酸组成的蛋白质，分子量为 28,930Da。活性蛋白酶 K 的特征是具有未水解的蛋白链和自溶的多肽链。

Application

在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
蛋白酶 K 催化角蛋白的水解。

Biochem/physiol Actions

蛋白酶 K 催化角蛋白的水解。
蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp³⁹-His⁶⁹-Ser²²⁴)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

Features and Benefits

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Other Notes

在pH 7.5，37 °C 条件下，一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μmol (181μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。

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