产品介绍
Product Description
General description
dTTP is a 100 mM clear, colorless solution of lithium salt (pH 7).
dTTP solution in water (pH 7), 100 mM, lithium salt.
Application
Use dTTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.
It has been used in PCR fingrprinting and PCR techniques.
Analysis Note
Typical analysis:
- ≥98% dTTP (HPLC), ≤1.5% dTDP (HPLC), ≤0.5% dTMP (HPLC)
- Absorbance determination A250/A260, A280/A260, A290/A260
- DNase activity negative, according to the current Quality Control procedures
- Suitability for PCR
Other Notes
1 μmol dTTP = 9.5 A260units
For life science research only. Not for use in diagnostic procedures.
Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
- nucleotide sets (all 4 nucleotides in separate vials)
- ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)
They are also vital components of:
- kits (dNTPs, buffer, and polymerase in separate vials)
- ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]
技术参数
Specifications
| assay | ≥98% |
| form | solution |
| usage | sufficient for 6,250 standard reactions (PCR) |
| packaging | pkg of 250 μL (25 μmol) |
| manufacturer/tradename | Roche |
| concentration | 100 mM |
| technique(s) | DNA amplification: suitable,DNA sequencing: suitable |
| storage temp. | −20°C |
| SMILES string | [NaH].[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)C)O)(O[P](=O)(O)O)O |
| InChI | 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);; |
| InChI key | KAELIXTVSANDSE-UHFFFAOYSA-N |
