General description
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Immunogen
MCF-7 human breast adenocarcinoma cell line
Biochem/physiol Actions
The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Physical form
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | FITC conjugate |
| Quality Level | 100 |
| antibody product type | primary antibodies |
| clone | A53-B/A2, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | 1 mg/ml |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2a |
| UniProt accession no. | P08727 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... KRT19(3880) |
