General description
Annexin V is a member of the annexin family of calcium and membrane-binding proteins that have anticoagulatory and anti-inflammatory properties. The encoded protein is 320 amino acid long and is folded into four domains, each having five α-helices, forming a superhelical globular structure. The ANX5 gene is mapped to human chromosome 4q26-q28 and spans a length of 28kb containing 13 exons and 12 introns.
Application
Annexin V has been used to examine phosphorylation activity of ChaK1 (channel-kinase 1).
Biochem/physiol Actions
Annexins are a family of water-soluble proteins that bind to membranes and phospholipids in a calcium-dependent manner. They participate in several cellular functions, such as membrane fusion and cytoskeletal interaction. Annexin V is a potent anticoagulant and serves as a voltage-gated calcium channel when bound to membrane in vitro. It is found to inhibit phospholipase A2 and protein kinase C.
Physical form
Lyophilized from PBS, pH 7.4.
Preparation Note
Reconstitute with 100 μL deionized water.
Other Notes
Recombinant human annexin V fused to a GST-tag.
| biological source | human |
| recombinant | expressed in E. coli |
| tag | GST tagged |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized |
| mol wt | ~52 kDa |
| packaging | pkg of 100 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | activity assay: suitable |
| color | white |
| UniProt accession no. | P08758 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... ANXA5(308) |
