General description
Protein unc-93 homolog B1 (UniProt: Q9H1C4; also known as Unc-93B1, hUNC93B1) is encoded by the UNC93B1 (also known as UNC93, UNC93B) gene (Gene ID: 81622) in human. Unc-93B1 is a multi-pass membrane glycoprotein with twelve transmembrane domains. It is a key regulator of nucleic acid (NA)-sensing Toll-like receptors (TLRs) and plays a key role in innate and adaptive immunity. It is highly expressed in antigen-presenting cells. Its expression has been reported in plasmocytoid dendritic cells. Lower levels of expression are observed in heart, kidney, and other tissues. It is localized in endosomes, lysosomes, and endoplasmic reticulum (ER). It re-localizes from ER to endosome and lysosome upon cell-stimulation with CpG dinucleotides. It is thought to guide NA-sensing TLRs (TLR 3, 7, 9) from ER to their respective compartments. UNC-93B1-deficient cells are reported to be unresponsive to TLR3, TLR7, TLR8, and TLR9. Its deficiency is shown to cause a near-complete loss of TLR3 and TLR7 proteins in primary splenic mouse dendritic cells and macrophages. Mutations in UNC93B1 gene are known to cause infection-induced acute encephalopathy and predisposes otherwise healthy subjects to herpes simplex encephalitis due to impaired interferon production. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pelka, K., et al., 2018, Immunity 48(5); 911 922; Isnardi, I., et al. (2008). Immunity. 29(5); 746-757).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Protein unc-93 homolog B1 (UNC93B1).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected UNC93B1 in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected UNC93B1 in A549 cell lysate and Raji cell membrane extract.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected UNC93B1 in A549 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected UNC93B1 in human small intestine tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound UNC93B1 peptide with a KD of 9.9 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected UNC93B1 in one million A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected UNC93B1 in HeLa cell lysate.
Biochem/physiol Actions
Clone 2C13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Protein unc-93 homolog B1 (UNC93B1). It targets an epitope within 19 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2C13, recombinant monoclonal |
| description | 2C13 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 66.63 kDa,observed mol wt ~80 kDa |
| purified by | using Protein A |
| species reactivity | bovine, human |
| species reactivity (predicted by homology) | bovine, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,affinity binding assay: suitable,flow cytometry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_112192 |
| UniProt accession no. | Q9H1C4 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... UNC93B1(81622) |
