Biochem/physiol Actions
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.
Physical form
Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.
Preparation Note
Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.
Analysis Note
The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.
| biological source | rat |
| Quality Level | 200 |
| recombinant | expressed in E. coli |
| assay | >97% (SDS-PAGE) |
| form | buffered aqueous solution |
| potency | 0.01-0.06 ng/mL ED50 |
| quality | endotoxin tested |
| mol wt | predicted mol wt ~22 kDa |
| packaging | pkg of 10 and 50 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.01EU/mg (LAL test) |
| UniProt accession no. | P20607.1 |
| shipped in | dry ice |
| storage temp. | −70°C |
