产品介绍
Product Description
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.
技术参数
Specifications
| biological source | human |
| recombinant | expressed in baculovirus infected insect cells |
| assay | ≥60% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 92 kDa |
| packaging | pkg of 10 μg |
| manufacturer/tradename | Sigma-Aldrich |
| concentration | >0.02 mg/mL |
| technique(s) | inhibition assay: suitable |
| solubility | water: soluble |
| NCBI accession no. | NM_005484 |
| UniProt accession no. | Q9UGN5 |
| application(s) | life science and biopharma |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PARP2(10038) |
