General description
CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine is a membrane protein, which is the only member of CXC3 family. Its chemokine domain (CKD) anchors it to the cell membrane through its mucin-like stalk. It acts as a ligand for the G-protein coupled receptor CX3CR1, which is found on smooth muscle cells, T-cells, natural killer (NK) cells and monocytes. Fractalkine is expressed on epithelial, endothelial and smooth muscle cells and neurons.
Biochem/physiol Actions
CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine, along with CX3CR1 is involved in various disorders such as atherosclerosis, multiple sclerosis, neuropathic pain and rheumatic disorders. It facilitates the proliferation and survival of primary human vascular smooth muscle cells in an epidermal growth factor receptor (EGR)-dependent manner. It plays an essential role in cell survival and promotes the survival of cells such as monocytes, T-cells and microglia. In humans, it is thought to be involved in atherogenesis, and thus, might have potential as a target in treatment of cardiovascular disease. In patients with sickle cell disease (SCD), the serum levels of this chemokine is increased, thus implicating it in the pathogenesis of inflammation associated with SCD.
Leukocyte chemoattractant protein and member of the chemokine superfamily possessing a CX3C motif. Mouse fractalkine is chemotactic for neutrophils and T-lymphocytes but not monocytes.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 1.25 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to chemoattract freshly isolated peripheral blood lymphocytes.
| biological source | mouse |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.3-3 μg/mL |
| mol wt | calculated mol wt ~9.5 kDa |
| packaging | pkg of 25 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| impurities | endotoxin, tested |
| UniProt accession no. | O35188 |
| storage temp. | −20°C |
| Gene Information | mouse ... Cx3cl1(20312) |
