General description
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen
A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1).
Physical form
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Preparation Note
Store at -20℃. Avoid freeze / thaw cycles.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | 96-111 |
| species reactivity | rat, human, mouse |
| concentration | 1.74 mg/ml |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q96PU5 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer448) |
| Gene Information | human ... NEDD4L(23327) |
