General description
Cholinergic receptor muscarinic 3 (CHRM3) gene is located on the human chromosome at 1q43 and encodes for the M3 subtype of muscarinic acetylcholine (ACh) receptors. Muscarinic receptor 3 (M3R) is one of the five muscarinic receptors (M1R-M5R). It is a member of the class A family of G-protein coupled receptors (GPCRs). M3R is localized to the salivary glands, lachrymal glands, trachea and bronchi.
Immunogen
synthetic peptide corresponding to amino acid residues 461-479 of the third intracellular loop of rat muscarinic acetylcholine receptor M3. This sequence is identical in human, mouse, pig, and bovine.
Application
Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit has been used in immunohistochemistry and western blotting.
Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody is used to tag muscarinic acetylcholine receptor (M3) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of muscarinic acetylcholine receptor (M3) in information processing and plastic changes in CNS function.
Biochem/physiol Actions
Muscarinic receptor 3 (M3R) is involved in the contraction of the detrusor muscle. It is also involved in the secretion of saliva and bladder contraction. It participates in several pathological processes of lung disease, such as chronic obstructive pulmonary disease (COPD). Mutations in the CHRM3 gene is associated with congenital bladder malformations related to prune-belly-like syndrome (PBS).
Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody recognizes rat, human, mouse Muscarinic Acetylcholine Receptor (M3).
Physical form
Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized powder |
| species reactivity | mouse, rat, human |
| technique(s) | western blot: 1:200 using rat brain lysate |
| UniProt accession no. | P20309 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CHRM3(1131) mouse ... Chrm3(12671) rat ... Chrm3(24260) |
