General description
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at ′Ser-75′. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Immunogen
A synthesized peptide derived from human Phospho-RAF1 (S43)
Physical form
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation Note
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Other Notes
24-60aa
| recombinant | expressed in HEK 293 F cells |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody product type | primary antibodies |
| clone | 1F7, recombinant monoclonal |
| form | liquid |
| mol wt | 150 kDa |
| purified by | affinity chromatography |
| species reactivity | human |
| concentration | 1.05mg/ml |
| technique(s) | ELISA: 2000:10000,flow cytometry: 1:50-1:200,immunofluorescence: 1:50-1:200,immunohistochemistry: 1:50-1:200 |
| isotype | IgG |
| UniProt accession no. | P04049 |
| shipped in | wet ice |
| storage temp. | -10 to -25°C |
| target post-translational modification | phosphorylation (pSer43) |
