General description
The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin
Application
The reagent is designed for Flow Cytometry analysis of cells expressing HLA-G molecule on the cell surface using a 1:500 dilution. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Features and Benefits
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Physical form
Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | biotin conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 87G, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2a |
| NCBI accession no. | NM_002127.5 |
| UniProt accession no. | P17693 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... HLA-G(3135) |
