产品介绍
Product Description
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
Application
RPMI-1640 Medium has been used
- to obtain spleen and lymph nodes from mice
- for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
- in the isolation of hepatitis B surface antigen-specific B-cell clones
Preparation Note
Supplement with 0.3 g/L
技术参数
Specifications
| Quality Level | 400 |
| sterility | sterile-filtered |
| form | liquid |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| pH | >7.2 |
| web metadata keyword.default | classic media, classical media, FBS, lymphoblastoid cells, cell fusion, hybrid cells |
| components | sodium pyruvate: no HEPES: 20 mM L-glutamine: no NaHCO3: yes phenol red: yes |
| shipped in | ambient |
| storage temp. | 2-8°C |
