Application
Prostaglandin F synthase (PGF synthase) is a member of the aldo-keto reductase superfamily of proteins which catalyze the conversion of aldehydes and ketones to thier corresponding alcohols. Prostaglandin F synthase is used in cancer research since it is upregulated in many cancers such as breast and prostate cancer. PGF synthase is also used to study kidney and renal cell carcinoma .
Biochem/physiol Actions
PGF synthase catalyzes the conversions of 4-androstene-3,17-dione to testosterone, estrone to 17β-estradiol, prostaglandin H2 to PGF2α, and PGD2 to 9α,11β-PGF2. Prostaglandin (PG) F2 is synthesized via three pathways .
Other Notes
One unit is defined as the amount of enzyme required to produce 1 μmol of NADP per minute at 37°C in 50 mM KPO4 pH 7.2 containing 250 μM NADPH and 25 μM 9,10-phenanthrenequinone
| recombinant | expressed in E. coli |
| Quality Level | 200 |
| description | contains a C-terminal hexahistidine tag |
| assay | ≥90% (SDS-PAGE) |
| form | liquid |
| specific activity | ≥0.4 μmol/mg protein |
| mol wt | ~37 kDa |
| NCBI accession no. | NP_003730 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... AKR1C3(8644) |
