General description
Anti-Monkeypox virus/MPXV M1R Antibody, clone SAA0284 is a monoclonal antibody produced in mouse. The monoclonal antibodies are IgG immunoglobulins, composed of two heavy chains and two light chains. Each chain contains a variable region that is specific for the target antigen, and a constant region that is responsible for binding to the B-cell receptor and other molecules involved in the immune response.
Monkeypox virus (MPXV), like smallpox virus, belongs to the genus Orthopoxvirus. Its morphology is consistent with that of orthopoxvirus, with a brick-shaped or oval shape with rounded corners and a size of 200~400nm. Brick-shaped or oval-shaped, covered by a lipoprotein membrane or envelope, with two protein-containing side bodies in the middle, and a thick membrane core containing a large double-stranded DNA gene body. The genome of Mpox virus is a double-stranded DNA, about 197kb long, and the end of the genome contains an identical but opposite terminal inverted repeat sequence. The virus contains 190 open reading frames, 4 of which are located in terminal inverted repeats. The core proteins of monkeypox virus are A29L, A35R, B6R, M1R, H3L.
Application
ELISA,neutralization
Biochem/physiol Actions
Monoclonal Anti-Monkeypox virus/MPXV M1R Antibody, clone SAA0284 binds to the surface of the monkeypox virus, preventing it from entering the cells of the host organism. This prevents the virus from replicating and spreading, thus providing protection against infection.
Packaging
100µg per vial / 1 mg per vial
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.
| biological source | mouse |
| Quality Level | 100 |
| recombinant | expressed in CHO cells |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| clone | SAA0284, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | liquid |
| mol wt | 150 kDa |
| species reactivity | monkeypox virus |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:2000-1:4000,neutralization: 0.01-10 μg/mL |
| color | colorless |
| pH | 7.4 |
| isotype | IgG2a, kappa |
| Protein ID accession no. | NP_536507.1 |
| UniProt accession no. | Q8V502 |
| shipped in | wet ice |
| storage temp. | -65 to -96°C |
| target post-translational modification | unmodified |
