General description
There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM ) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.
Application
IgM是一种糖蛋白，重链上具有5个n连接的糖基化位点。可通过ELISA分析确定结合凝集素的IgM的糖基化形式，其中碱性磷酸酶标记的山羊抗人IgM可作为二抗（1:2000稀释），以对硝基苯基为底物(Sigma)。
IgM是一种糖蛋白，重链上有5个n连接糖基化位点。可通过ELISA分析确定结合凝集素的IgM的糖基化形式，其中碱性磷酸酶标记的山羊抗人IgM可作为二抗（1:2000稀释），以对硝基苯基为底物(Sigma)。Arnold, J. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin.JBC, 280: 29080-29087.
Biochem/physiol Actions
Goat polyclonal anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones kappa, and lambda myeloma proteins. The conjugate shows no reactivity with mouse or rat IgG.
Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Other Notes
No cross-reaction with mouse and rat IgG
Legal Information
CDP-Star is a registered trademark of Tropix, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Level | 200 |
| conjugate | alkaline phosphatase conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| technique(s) | direct ELISA: 1:50,000,dot blot: 1:60,000,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200,western blot (chemiluminescent): 1:60,000 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
