Biochem/physiol Actions
WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
| biological source | human |
| recombinant | expressed in insect cells |
| assay | ≥60% (SDS-PAGE) |
| form | frozen liquid |
| packaging | pkg of 5 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| concentration | 150 μg/mL |
| color | clear colorless |
| NCBI accession no. | NM_024425 |
| UniProt accession no. | P19544 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... WT1(7490) |
