General description
The gene IGFBP-5 (insulin-like growth factor binding protein-5) is mapped to human chromosome 2q35.
Biochem/physiol Actions
Binds to bone cells and is the predominant insulin-like growth factor binding protein in bone; when binding the extracellular matrix, it modulates IGF activity.
IGFBP-5 (insulin-like growth factor binding protein-5) interacts with IGFs and thereby controls IGF activity. High levels of IGFBP5 are present in systemic sclerosis and idiopathic pulmonary fibrosis conditions. It also plays a crucial role in cancer progression.
IGFBP-5 binds to bone cells and is the predominant insulin-like growth factor binding protein in bone. When IGFBP-5 binds the extracellular matrix, it modulates IGF activity. Mouse, human, and rat IGFBP-5 share 97% homology.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA.
Analysis Note
The biological activity is measured by its ability to inhibit recombinant human IGF-1 or IGF-II on MCF-7 cells.
| biological source | human |
| Quality Level | 100 |
| recombinant | expressed in NSO cells |
| assay | >90% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.5-1.5 mg per mL ED50 |
| mol wt | 34 kDa by SDS-PAGE (reducing),calculated mol wt ~28 kDa |
| packaging | pkg of 25 μg |
| impurities | endotoxin, tested |
| UniProt accession no. | P24593 |
| storage temp. | −20°C |
| Gene Information | human ... IGFBP5(3488) |
