General description
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP8 plays a role in viral RNA synthesis (3,4,5). Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, it may synthesize substantially longer products than oligonucleotide primers.
Immunogen
Anti-SARS-CoV-2 (COVID-19) NSP8 antibody was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP8 protein. The immunogen is located within 60-110 amino acids of SARS-CoV-2 (COVID-19) NSP8.
Application
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP8 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP8 recombinant protein by ELISA. All other applications and species not yet tested.
Physical form
Supplied in PBS containing 0.02% sodium azide.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| species reactivity | SARS coronavirus |
| concentration | 1 mg/mL |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable |
| isotype | IgG |
| NCBI accession no. | YP_009742615.1 |
| UniProt accession no. | P0DTC1 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | SARS coronavirus ... ORF1ab(43740578) |
