General description
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Immunogen
Activated C57BL/6 mouse spleen cells
Biochem/physiol Actions
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Physical form
Phosphate buffered saline (PBS), pH 7.4
Preparation Note
Purified by protein-G affinity chromatography.
Store at 2-8°C. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
| conjugate | unconjugated |
| Quality Level | 100 |
| antibody product type | primary antibodies |
| clone | M5/114, monoclonal |
| form | buffered aqueous solution |
| species reactivity | mouse |
| concentration | 1 mg/ml |
| technique(s) | immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable,FUNC: suitable,flow cytometry: suitable |
| isotype | IgG2b |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
