General description
Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
Application
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Features and Benefits
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
Legal Information
Sephadex is a registered trademark of Cytiva
| grade | Molecular Biology |
| Quality Level | 200 |
| product line | BioReagent |
| form | beads |
| swelling | 1 g swells to 9-11 mL |
| bead diameter | 50-150 μm |
| pore size | exclusion limit (20 bp dsDNA; (rA)20) |
| pH | 2-10 |
| foreign activity | DNase, RNase, none detected |
| storage temp. | room temp |
| SMILES string | O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O |
| InChI | 1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2 |
| InChI key | FZWBNHMXJMCXLU-UHFFFAOYSA-N |
