General description
DYRK3 or dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 is belongs to the DYRK family of dual-specificity protein kinases that catalyze autophosphorylation on serine/threonine and tyrosine residues. DYRK3 expressed in E. coli undergoes tyrosine autophosphorylation and catalyzes phosphorylation of histones H3 and H2B in vitro. DYRK3 regulate different steps of the caveolar cycle. DYRK3 can promote cell survival through phosphorylation and activation of SIRT1. DYRK3 directly phosphorylate SIRT1 at Thr(522), promoting deacetylation of p53.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | human |
| recombinant | expressed in E. coli |
| product line | PRECISIO® |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 194-290 nmol/min·mg |
| mol wt | ~95 kDa |
| technique(s) | cell culture | mammalian: suitable |
| NCBI accession no. | NM_001004023 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... DYRK3(8444) |
