产品介绍
Product Description
General description
MegaCell™ is a multifaceted media developed to reduce the amount of serum required for culturing mammalian cells in vitro. It reduces the supplementation of fetal bovine serum (FBS) or other serum from 10% to 3% and supports proliferative rates and maximal cell densities. MegaCell enables maintaining a long-term culture of non-fastidious cell lines.
Application
MegaCell™ Dulbecco′s Modified Eagle′s Medium has been used:
- as a component of the M10 medium to culture CD133+ cells from biopsies of para-spinal and quadriceps muscle of Duchenne muscular dystrophy patients
- as a component of the proliferation medium for the expansion and cryopreservation of human induced pluripotent stem (iPS) cells cultured without feeder cells and human embryonic (ES) cell-derived mesoangioblast-like cells
- to culture the positive hybridomas during the production of mouse anti-chicken CD25 monoclonal antibodies
Preparation Note
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
Legal Information
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
技术参数
Specifications
| sterility | sterile-filtered |
| Quality Level | 300 |
| form | liquid |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| web metadata keyword.default | Reduced serum usage, FBS, fetal bovine serum reduced use |
| components | L-glutamine: no glucose: 4500 mg/L (Dextro) sodium pyruvate: yes |
| shipped in | ambient |
| storage temp. | 2-8°C |
