General description
Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome.
Immunogen
recombinant human Aurora-A kinase.
Application
Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry.
Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.
Biochem/physiol Actions
Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans .
Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr15 by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser353. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis.
Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in mouse cell line |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 35C1, monoclonal |
| form | PBS solution |
| mol wt | antigen ~46 kDa |
| species reactivity | human, mouse |
| concentration | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,indirect ELISA: suitable,western blot: 0.25-0.5 μg/mL using HeLa total cell extract |
| isotype | IgG2b |
| UniProt accession no. | O14965 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... AURKA(6790) mouse ... Aurka(20878) |
