General description
Affinity purification of GST fusion proteins
The BugBuster® GST•Bind Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.
Other Notes
| • | 2 x 100 ml | BugBuster Protein Extraction Reagent
|
| • | 10,000 U | Benzonase Nuclease, purity >90%
|
| • | 10 ml | GST•Bind Resin
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| • | pkg/4 | Chromatography Columns
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| • | 2 x 100 ml | 10X GST Bind/Wash Buffer
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| • | 40 ml | 10X Glutathione Reconstitution Buffer
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| • | 1 g | Glutathione, Reduced |
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
